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Immune responses of Asian sea bass, Lates calcarifer Bloch, against an inactivated betanodavirus vaccine
Author(s) -
Pakingking Jr R,
Seron R,
Dela Peña L,
Mori K,
Yamashita H,
Nakai T
Publication year - 2009
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2009.01040.x
Subject(s) - lates , sea bass , biology , virology , titer , virus , grouper , antibody , vaccination , immune system , fish <actinopterygii> , immunology , fishery
Asian sea bass, Lates calcarifer (Bloch), exhibited strong immune responses against a single injection of the formalin‐inactivated red‐spotted grouper nervous necrosis virus (RGNNV), a betanodavirus originally isolated in Japan. Fish produced neutralizing antibodies at high titre levels from days 10 (mean titre 1:480) to 116 (1:1280), with the highest titre at day 60 post‐vaccination (1:4480). When fish were challenged with the homologous RGNNV at day 54 post‐vaccination, there were no mortalities in both the vaccinated and unvaccinated control fish. However, a rapid clearance of the virus was observed in the brains and kidneys of vaccinated fish, followed by a significant increase in neutralizing‐antibody titres. Furthermore, the vaccine‐induced antibodies potently neutralized Philippine betanodavirus isolates (RGNNV) in a cross‐neutralization assay. The present results indicate the potential of the formalin‐inactivated RGNNV vaccine against viral nervous necrosis (VNN) of Asian seabass.