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Genetic characterization of Streptococcus phocae strains isolated from Atlantic salmon, Salmo salar L., in Chile
Author(s) -
Valdés I,
Jaureguiberry B,
Romalde J L,
Toranzo A E,
Magariños B,
AvendañoHerrera R
Publication year - 2009
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2008.01014.x
Subject(s) - biology , salmo , rapd , pulsed field gel electrophoresis , intergenic region , typing , genetics , microbiology and biotechnology , genetic diversity , dna profiling , genotype , polymerase chain reaction , outbreak , virology , dna , gene , genome , fishery , population , demography , sociology , fish <actinopterygii>
Streptococcus phocae is a beta‐haemolytic bacterium frequently involved in disease outbreaks in seals causing pneumonia or respiratory infection. Since 1999, this pathogen has been isolated from diseased Atlantic salmon, Salmo salar , causing serious economic losses in the salmon industry in Chile. In this study, we used different molecular typing methods, such as pulsed‐field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus sequence PCR (ERIC‐PCR), repetitive extragenic palindromic PCR (REP‐PCR) and restriction of 16S–23S rDNA intergenic spacer regions to evaluate the genetic diversity in S. phocae . Thirty‐four strains isolated in different years were analysed. The S. phocae type strain ATCC 51973 T was included for comparative purposes. The results demonstrated genetic homogeneity within the S. phocae strains isolated in Chile over several years, suggesting the existence of clonal relationships among S. phocae isolated from Atlantic salmon. The type strain ATCC 51973 T presented a different genetic pattern with the PFGE, RAPD, ERIC‐PCR and REP‐PCR methods. However, the fingerprint patterns of two seal isolates were distinct from those of the type strain.