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Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines
Author(s) -
Anderson E,
Clouthier S,
Shewmaker W,
Weighall A,
LaPatra S
Publication year - 2008
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2008.00960.x
Subject(s) - infectious hematopoietic necrosis virus , virology , biology , rainbow trout , virus , infectivity , antigen , microbiology and biotechnology , antibody , vaccination , immunology , fishery , fish <actinopterygii>
The inactivation dynamics of infectious haematopoietic necrosis virus (IHNV) by β‐propiolactone (BPL), binary ethylenimine (BEI), formaldehyde or heat and the antigenic and immunogenic properties of the inactivated vaccines were evaluated. Chemical treatment of IHNV with 2.7 m m BPL, 1.5 m m BEI or 50 m m formaldehyde abolished virus infectivity within 48 h whereas heat treatment at 50 or 100 °C rendered the virus innocuous within 30 min. The inactivated IHNV vaccines were recognized by rainbow trout, Oncorhynchus mykiss , IHNV‐specific antibodies and were differentially recognized by antigenic site I or antigenic site II IHNV glycoprotein‐specific neutralizing monoclonal antibodies. The BPL inactivated whole virus vaccine was highly efficacious in vaccinated rainbow trout challenged by waterborne exposure to IHNV 7, 28, 42 or 56 days (15 °C) after immunization. The formaldehyde inactivated whole virus vaccine was efficacious 7 or 11 days after vaccination of rainbow trout but performed inconsistently when tested at later time points. The other vaccines tested were not efficacious.