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Expression and immunogenic comparison of VP2 and VP3 from marine birnavirus
Author(s) -
Xu HJ,
Lin JG,
Zhang CX,
Suzuki S
Publication year - 2008
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2008.00909.x
Subject(s) - polyclonal antibodies , biology , fusion protein , complementary dna , escherichia coli , microbiology and biotechnology , glutathione s transferase , antibody , recombinant dna , epitope , glutathione , virology , gene , biochemistry , enzyme , immunology
The coding regions for the major epitopes of structural protein VP2 ( vp2e ) and structural protein VP3 were amplified from marine birnavirus (MABV) cDNA and efficiently expressed as glutathione S‐transferase (GST) fusion proteins in Escherichia coli . Polyclonal antibodies against VP2e and VP3 were raised in rabbits and fish using the purified proteins of GST/VP2e and GST/VP3. The rabbit anti‐serum against VP3 was more sensitive than the rabbit anti‐VP2e serum in detecting virus in MABV‐infected fish, while fish anti‐VP2e serum showed a stronger neutralization response than fish anti‐VP3 serum.