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gyrA and parC associated with quinolone resistance in Vibrio anguillarum
Author(s) -
Rodkhum C,
Maki T,
Hirono I,
Aoki T
Publication year - 2008
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2007.00843.x
Subject(s) - marine biology , genome , library science , biology , engineering ethics , engineering , ecology , genetics , computer science , gene
Keywords: gyrA, gyrB, parC, parE, quinolone resis-tance, Vibrio anguillarum.Vibrio anguillarum is a Gram-negative comma-shaped bacterium with polar agella that is amember of the family Vibrionaceae. It is the cause ofvibriosis or haemorrhagic septicaemic disease inwild and cultured marine and freshwater sh (Actis,Tolmasky & Crosa 1999; Austin & Austin 1999).Chemotherapeutic agents, including quinolones,have been widely used for the treatment of vibriosisin cultured sh and multiple drug-resistant strainsof V. anguillarum have subsequently arisen (Aoki1992; Pedersen, Tiainen & Larsen 1995; WorldHealth Organization 1999). Drug-resistance geneson the transferable R-plasmid have been previouslycharacterized, but the R-plasmid of V. anguillarumdoes not carry any genes for quinolone resistance(Aoki, Egusa & Arai 1974). The mutations in genesand amino acids associated with quinolone resis-tance in V. anguillarum are still unknown, and itwould therefore be useful to characterize thesemutations.There are two functional domains related toquinolone resistance in Gram-negative bacteria:DNA gyrase and topoisomerase IV (Hooper2000). DNA gyrase is composed of two subunits,gyrA and gyrB, whereas topoisomerase IV comprisestwo subunits, parC and parE. Topoisomerase IVhas DNA decatenating and relaxing activities, andplays an essential role in partitioning chromosomesat the terminal stage of chromosome replication(Huang 1996; Hooper 1999, 2000). The molecularmechanisms of quinolone and uoroquinoloneresistance have been described in several organisms(Hooper & Wolfson 1993; Hooper 2000; Chen L Okuda, Hayakawa, Nishibu-chi & Nishino 1999; Ozanne, Benveniste, Tipper& Davies 2005). To understand the mechanism ofquinolone resistance in V. anguillarum, the nucle-otide sequences of the gyrA, gyrB, parC and parEgenes were sequenced and characterized in thisstudy.Twenty-ve wild V. anguillarum strains isolatedfrom cultured ayu, Plecoglossus altivelis (Temminck& Schlegel), in Japan, and V. anguillarum strainH775-3 were used (Table 2). The 25 wildstrains included 10 oxolinic acid (OA)-sensitivestrains [minimal inhibitory concentration (MIC):<0.4 lgmL