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RT‐PCR amplification and sequence analysis of extra small virus associated with white tail disease of Macrobrachium rosenbergii (de Man) cultured in Taiwan
Author(s) -
Wang C S,
Chang J S,
Shih H H,
Chen S N
Publication year - 2007
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2007.00793.x
Subject(s) - macrobrachium rosenbergii , biology , virus , phylogenetic tree , polymerase chain reaction , virology , prawn , white spot syndrome , shrimp , sequence analysis , rapid amplification of cdna ends , nucleic acid sequence , homology (biology) , gene , complementary dna , genetics , microbiology and biotechnology , molecular cloning , fishery
Post‐larvae of Macrobrachium rosenbergii infected with white tail disease (WTD) have been reported in Taiwan. The causative agents have been identified as M. rosenbergii nodavirus ( Mr NV) associated with extra small virus (XSV). The present study is the first report confirming the presence of XSV virus in M. rosenbergii displaying WTD symptoms in Taiwan by reverse transcription polymerase chain reaction (RT‐PCR). A 772 bp amplified product was obtained by RT‐PCR, cloned and sequenced. The nucleotide sequence analysis of the 772 bp DNA fragment revealed 98% and 97% identity with XSV isolated from China and India, respectively. Comparison of the deduced amino acid sequences of the XSV partial genome showed strong homology (99% and 97%) with isolates from China and India. Phylogenetic analysis revealed the XSV‐Taiwan isolate was more closely related to the Chinese rather than the Indian isolates. The results demonstrated the presence of XSV virus co‐infection in M. rosenbergii cultured in Taiwan suffering from WTD.