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Presumptive detection of yellow head virus by reverse transcriptase‐polymerase chain reaction and dot‐blot hybridization in Litopenaeus vannamei and L. stylirostris cultured on the Northwest coast of Mexico
Author(s) -
De La RosaVélez J,
CedanoThomas Y,
CidBecerra J,
MéndezPayán J C,
VegaPérez C,
ZambranoGarcía J,
Bonami JR
Publication year - 2006
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2006.00767.x
Subject(s) - shrimp , biology , polymerase chain reaction , virology , virus , amplicon , reverse transcriptase , infectivity , dot blot , bioassay , microbiology and biotechnology , veterinary medicine , gene , fishery , ecology , genetics , medicine
In order to assess the presence of yellow head virus (YHV) in shrimp farms along the Pacific coast of Mexico, 39 samples from 26 randomly chosen farms were analysed by means of reverse transcriptase‐polymerase chain reaction (RT‐PCR) and dot‐blot hybridization. Eleven samples were positive for YHV. The disease was reproduced by means of an infectivity bioassay performed with an extract of pleopods from the positive samples. Cumulative mortality reached 50% in 14 days. Four pairs of primers which amplified several YHV genome regions were designed and used to test dead and surviving shrimp from the bioassay by RT‐PCR, resulting in positive results for every expected amplicon. The results of this study provide presumptive evidence of the presence of YHV in Mexican shrimp farms at least during 1999–2000.