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Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool
Author(s) -
McBeath S J,
Ellis L M,
Cook P F,
Wilson L,
Urquhart K L,
Bricknell I R
Publication year - 2006
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2006.00720.x
Subject(s) - polyclonal antibodies , antiserum , biology , virology , virus , immunology , antibody
Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase‐based immunoassay, and Western blot.