Premium
Putative virulence‐related genes in Vibrio anguillarum identified by random genome sequencing
Author(s) -
Rodkhum C,
Hirono I,
Stork M,
Lorenzo M,
Crosa J H,
Aoki T
Publication year - 2006
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2006.00692.x
Subject(s) - biology , gene , genetics , cosmid , genome , vibrio anguillarum , virulence , homology (biology) , gene cluster , plasmid , whole genome sequencing , vibrio , bacteria
The genome of Vibrio anguillarum strain H775‐3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence‐related genes were identified, 36 of which are novel in V. anguillarum , including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes.