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Engulfed pathogen‐induced apoptosis in haemocytes of giant freshwater prawn, Macrobrachium rosenbergii
Author(s) -
Hsu JP,
Huang C,
Liao CM,
Hsuan SL,
Hung HH,
Chien MS
Publication year - 2005
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2005.00681.x
Subject(s) - macrobrachium rosenbergii , biology , microbiology and biotechnology , aeromonas hydrophila , dna laddering , phagocytosis , tunel assay , prawn , apoptosis , pathogen , dna fragmentation , vacuole , acridine orange , bacteria , biochemistry , cytoplasm , programmed cell death , fishery , genetics
Haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii , were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non‐pathogenic latex beads in vitro . Isolated haemocytes of M. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast Debaryomyces hansenii , the bacteria Aeromonas hydrophila or Enterococcus faecium , or with latex beads at 25 °C for 2 h, followed by washing to remove free particles. At least 200 haemocytes were counted to determine the phagocytosis rate, and the results showed that haemocytes engulfed latex beads at a higher rate than the aquatic pathogens. By transmission electron microscopy, the yeast‐ or bacterium‐engulfing haemocytes displayed morphological changes characteristic of apoptosis, including formation of cytoplasmic vacuoles, chromatin condensation and fragmentation of nuclei. This pathogen‐induced apoptosis was further confirmed by DNA laddering and TUNEL (terminal deoxynucleotidyl transferase‐mediated deoxy‐UTP nick‐end‐labelling) assays. Neither haemocytes treated with latex beads nor uninfected haemocytes (control group) showed signs of apoptosis after 48 h in culture.