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Loop‐mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens
Author(s) -
Savan R,
Kono T,
Itami T,
Sakai M
Publication year - 2005
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2005.00670.x
Subject(s) - loop mediated isothermal amplification , biology , aquaculture , white spot syndrome , shellfish , bacterial disease , microbiology and biotechnology , fishery , virus , virology , aquatic animal , fish <actinopterygii> , dna , genetics
Fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. Early diagnosis plays a vital role in management of fish and shellfish diseases. Traditionally, various biochemical and serological tests have been used for fish disease diagnosis. However, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. Polymerase chain reaction and probe‐based nucleic acid detection have become increasingly popular in fish and shellfish diagnostics. Recently, a novel technique called loop‐mediated isothermal amplification (LAMP) has been developed, which is highly sensitive and rapid. LAMP has been used for the detection of bacterial, viral, fungal and parasitic diseases in both animal and plants. In aquaculture, LAMP‐based detection of pathogens like Edwardsiella tarda , E. ictaluri , Nocardia seriolae , Tetracapsuloides bryosalmonae , white spot syndrome virus and infectious haematopoietic necrosis virus have been reported. In this review, the application of LAMP for the detection of aquaculture‐associated pathogens is discussed.