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Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine
Author(s) -
Temprano A,
Riaño J,
Yugueros J,
González P,
Castro L,
Villena A,
Luengo J M,
Naharro G
Publication year - 2005
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2005.00646.x
Subject(s) - aroa , yersinia ruckeri , biology , microbiology and biotechnology , mutant , virology , attenuated vaccine , vaccination , gene , rainbow trout , enterobacteriaceae , genetics , virulence , fish <actinopterygii> , escherichia coli , fishery
The aroA gene of Yersinia ruckeri , which encodes 5‐enolpyruvylshikimate 3‐phosphate synthase, was insertionally inactivated with a DNA fragment containing a kanamycin resistance determinant and reintroduced by allelic exchange into the chromosome of Y. ruckeri 21102 O1 by means of the suicide vector pIVET8. The Y. ruckeri aroA ::Kan r mutant was highly attenuated when inoculated intraperitoneally into rainbow trout, with a 50% lethal dose of >5 × 10 7 CFU. The mutants were not recoverable from the internal organs 48 h post‐inoculation or later. The vaccination of rainbow trout with the AroA mutant as a live vaccine conferred significant protection (relative percentage survival = 90%) against the pathogenic wild‐type strain of Y. ruckeri.