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Isolation of lymphocystis disease virus from sole, Solea senegalensis Kaup, and blackspot sea bream, Pagellus bogaraveo (Brünnich)
Author(s) -
Alonso M C,
Cano I,
GarciaRosado E,
Castro D,
Lamas J,
Barja J L,
Borrego J J
Publication year - 2005
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2005.00621.x
Subject(s) - biology , strain (injury) , polymerase chain reaction , cytopathic effect , microbiology and biotechnology , fishery , virus , virology , anatomy , gene , genetics
Two viruses were isolated from cultured sole, Solea senegalensis , and wild blackspot sea bream, Pagellus bogaraveo , and preliminarily characterized as lymphocystis disease viruses (LCDVs). Viral isolates were characterized by morphological, biochemical and biophysical properties. In addition, the susceptibility of four fish cell lines was also tested. LCDV isolates developed cytopathic effects on the SAF‐1 cell line at 5 and 6 days post‐infection and reached titres of 10 6 TCID 50 mL −1 . The antigenic and structural protein analysis of the two new LCDV isolates showed identical profiles to that obtained for LCDV strain Leetown NFH (ATCC VR‐342), used as a reference viral strain, and for an LCDV isolate collected from gilt‐head sea bream, Sparus aurata , cultured in southern Spain. Molecular confirmation was performed by polymerase chain reaction. Specific primers for LCDV produced a 270‐bp DNA fragment, the expected size for LCDV.