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Induction of apoptosis in a flounder gill cell line by lymphocystis disease virus infection
Author(s) -
Hu GB,
Cong RS,
Fan TJ,
Mei XG
Publication year - 2004
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2004.00588.x
Subject(s) - biology , dna laddering , apoptosis , flounder , iridovirus , dna fragmentation , virus , cytopathic effect , multiplicity of infection , virology , programmed cell death , cell culture , microbiology and biotechnology , fish <actinopterygii> , fishery , genetics
Lymphocystis disease virus (LCDV), a large icosahedral DNA virus classified to the iridovirus family, is the causative agent of lymphocystis, a disease which occurs in marine and freshwater fish species and is characterized by formation of papilloma‐like lesions on the surface of the skin. In vitro , LCDV infection causes flounder gill cells, an adherent cell line, to exhibit an obvious cytopathic effect (CPE). In order to test whether apoptosis is responsible for the observed CPE, cells infected with LCDV at a multiplicity of infection (m.o.i.) of 5 PFU per cell were examined at various time intervals for the appearance of apoptotic signs. Nuclear fragmentation, DNA laddering and caspase activation were observed in the infected cells at the time (i.e. 10 days post‐infection) when an intensive CPE was observed. These findings demonstrate that LCDV is capable of inducing apoptosis in vitro , which is different from the result of LCDV infection in vivo , and consequently suggest an intricate LCDV–host interaction.