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Immune responses and host protection of channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis after immunization with live theronts and sonicated trophonts
Author(s) -
Xu DH,
Klesius P H,
Shelby R A
Publication year - 2004
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.2004.00523.x
Subject(s) - ichthyophthirius multifiliis , ictalurus , catfish , biology , immune system , ictaluridae , antibody , immunity , intraperitoneal injection , immunization , immunology , fish <actinopterygii> , fishery , endocrinology
The humoral immune responses and host protection of channel catfish, Ictalurus punctatus (Rafinesque), against Ichthyophthirius multifiliis (Ich) were determined after immunization with live theronts and sonicated trophonts. Immunizations with live theronts or sonicated trophonts were carried out by both bath immersion and intraperitoneal (i.p.) injection. Cutaneous and serum immunoglobulin (Ig) levels and anti‐Ich antibodies were measured 12 and 21 days post‐immunization. The level of Ich infection and survival of catfish were determined after theront challenge. Cutaneous and serum anti‐Ich antibodies were significantly higher ( P < 0.05) in fish immunized with live theronts by immersion or i.p. injection, or with sonicated trophonts administered by i.p. injection, than in fish immunized with sonicated trophonts by immersion, with bovine serum albumin by i.p. injection, or non‐immunized controls. Host protection was noted only in fish immunized with live theronts by immersion or i.p. injection or with sonicated trophonts by i.p. injection. There was a positive correlation between higher levels of anti‐Ich antibodies and host survival in the immunized fish.