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Experiments with growth of the eel trypanosome, Trypanosoma granulosum Laveran & Mesnil, 1902, in semi‐defined and defined media
Author(s) -
DAVIES A. J.,
MASTRI C.,
THORBORN D. E.,
MACKINTOSH D.
Publication year - 1995
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1995.tb00364.x
Subject(s) - trypanosoma , biology , doubling time , in vitro , andrology , biochemistry , virology , medicine
. Growth of the eel trypanosome, Trypanosoma granulosum , was attempted in five semi‐defined and three defined media. Growth was poor in four semi‐defined and two defined media, but one semi‐defined medium, a modified version of SDM‐79 without MEM F‐14, supported good growth of the trypanosome. In this medium, doubling time was between 1 and 2 days, over 90% of organisms seen in culture after 6 days were trypomastigotes, and 1.8 × 0.1 × 10 7 trypanosomes ml −1 was achieved in 7 days. When foetal calf serum from the modified SDM‐79 was substituted with insulin to create a fully defined medium, a modest but significant increase in cell numbers occurred compared with controls. In vitro experiments with D, L‐alpha‐difluoromethylomithine (DFMO) showed morphological changes leading to destruction of the trypanosome with increasing concentrations of the drug. A 50‐mM concentration of DFMO inhibited growth by more than 90%, and the IC 50 was found to be 16 × 2 mM.