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Glycogen, the preferred energy source for extracellular protein formation and growth of Aeromonas salmonicida
Author(s) -
COLEMAN G.,
COLLIGHAN R. J.,
DODSWORTH S. J.
Publication year - 1995
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1995.tb00297.x
Subject(s) - maltose , glycogen , aeromonas salmonicida , biology , amylase , biochemistry , extracellular , incubation , food science , carbohydrate , energy source , microbiology and biotechnology , bacteria , enzyme , ecology , genetics , renewable energy
. A study was made of the effect of supplementing a rich 3% (w/v) tryptone soya broth (TSB) medium and a poorer 1.7% (w/v) tryptone‐based medium with glucose, maltose and glycogen, as carbon sources, on growth and exoprotein formation by Aeromonas salmonicida. In TSB, glucose inhibited growth and repressed exoprotein formation whilst maltose and glycogen had little effect, up to 20h, when compared with an unsupplemented control. By contrast, in the poorer medium, over a 24‐h incubation period, growth was stimulated three‐fold by glycogen, and whilst exoprotein formation was low in comparison with that observed in TSB, the greatest production was observed in the presence of glycogen. Extracellular α‐amylase was measured in the tryptone medium in the presence of the three carbon sources and the highest level, produced in the presence of glycogen, was 1.6 times that with added maltose whilst none was detectable with glucose present. This pattern was repeated in the case of the maltose‐inducible porin, LamB, of the outer membrane.