Premium
The route of entry and progression of infectious haematopoietic necrosis virus in Oncorhynchus mykiss (Walbaum): a sequential immunohistochemical study
Author(s) -
DROLET B. S.,
ROHOVEC J. S.,
LEONG J. C.
Publication year - 1994
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1994.tb00229.x
Subject(s) - biology , rainbow trout , infectious hematopoietic necrosis virus , gill , virology , virus , epizootic , immunohistochemistry , necrosis , haematopoiesis , microbiology and biotechnology , fishery , immunology , fish <actinopterygii> , stem cell , genetics
. Steelhead trout, Oncorhynchus mykiss (Walbaum), fry were experimentally infected with infectious haematopoietic necrosis virus (IHNV) Round Butte 1983 (Type 1). Fry were sampled daily, before and during the epizootic. Fish tissues were tested for infectious virus by tissue culture assay and for IHNV nucleocapsid protein by alkaline phosphatase immunohistochemistry (APIH). The progression of virus through the tissues was followed by APIH until the fourteenth day. Viral infection progressed from two major sites: from the gills into the circulatory system; and from the oral region into the gastrointestinal tract and then into the circulatory system. Once in the blood, virus was disseminated to virtually every organ. Progression of IHNV within and between organs is discussed.