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Elevated serum levels of alpha foetoprotein (AFP)‐like immunoreactivity in rainbow trout, Oncorhynchus mykiss (Walbaum), with aflatoxin B 1 induced hepatocellular carcinoma
Author(s) -
SARCIONE E. J.,
BLACK J. J.
Publication year - 1994
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1994.tb00217.x
Subject(s) - rainbow trout , hepatocellular carcinoma , biology , aflatoxin , trout , medicine , endocrinology , pathology , fish <actinopterygii> , fishery , cancer research , food science
. Alpha foetoprotein (AFP)‐like immunoreactivity was detected by double immunodiffusion in thesera of adult rainbow trout, Oncorhyachus mykiss (Walbaum) with histologically confirmed hepatocellular carcinoma experimentally induced in microinjection of embryos with aflatoxin B 1 (AFB 1 ). Partial characterization indicated that this AFP‐like immunoreactivity gave immunologic cross‐reaction of partial identity with human AFP. had alpha 1 electrophorctic mobility, and an estimated molecular weight of 72kDa. Elevated serum AFP‐like immunoreactivity levels were also measured by RIA in seven out of eight adult rainbow trout with hepatocellular carcinoma, and in three out of 17 AFB 1 ‐exposed trout without demonstrable grosws or microscopic evidence for hepatocellular neoplasms. No serum AFP‐like immunoreactivity was detected or measured in normal healthy age matched DMSO‐control rainbow trout. These data are consistent with the following conelusions: (1) AFP‐like immunoreactivity detected and measured in the sera of rainbow trout with hepaloecllular careinoma is an analogue to human AFP; and (2) the elevated serum AFP levels measured in adult rainbow trout wilh hepatocellular careinoma resrmble those found in humans with this same malignancy. These data further suggest that serum AFP measurements might be useful to confirm the appearance of hepatocellular carcinoma in experimental fish carcinogen‐assay systems, and to detect hepatocellular neoplasia in high‐risk wild fish populations exposed to carcinogenie pollutants.