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Comparative in vitro studies on virulent and avirulent strains of Cryptobia salmositica (Sarcomastigophora: Kinetoplastida)
Author(s) -
WOO P. T. K.,
THOMAS P. T.
Publication year - 1992
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1992.tb00662.x
Subject(s) - biology , virulence , microbiology and biotechnology , kinetoplastida , parasite hosting , antiserum , in vitro , virology , strain (injury) , protozoa , lytic cycle , virus , antibody , immunology , malaria , anatomy , protozoal disease , biochemistry , gene , world wide web , computer science
. The optimum temperature for in vitro multiplication of Cryptobia salmositica was 10°C. The avirulent strain multiplied more rapidly than the virulent strain. The haemolytic components, lytic component (LC) and immune complex‐forming component (ICC), were secreted by the two strains into the culture medium and were detectable from one week post‐inoculation. The haemolytic activity in the supernatant increased with increasing parasite numbers in both strains. Although cultures of the avirulent strain had higher parasite numbers than those of the virulent strain, the haemolytic activity was significantly lower than that of the virulent strain. Antiserum against ICC was produced in rabbit by immunization with ICC‐coated rainbow trout red blood cells.