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Ultrastructural changes associated with carbon tetrachloride hepatotoxicity in channel catfish, Ictalurus punctatus Rafinesque
Author(s) -
AFIFI S. H.,
MacMILLAN J. R.
Publication year - 1992
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1992.tb00646.x
Subject(s) - ictalurus , catfish , ultrastructure , carbon tetrachloride , endoplasmic reticulum , vacuole , biology , saline , juvenile , andrology , medicine , endocrinology , anatomy , biochemistry , chemistry , cytoplasm , fish <actinopterygii> , fishery , organic chemistry , genetics
. Ultrastructural changes occurring in juvenile channel catfish, Ictalurus punctatus Ratinesque, hepatocytes were examined following exposure to carbon tetrachloride (CCl 4 ). Juvenile catfish were intraperitoneally injected with a sublethal dose of undiluted CCl 4 and controls, used for comparisons, received physiological saline. The rough endoplasmic rcticulum (RER) was slightly dilated by 1 h and was significantly dilated by 4 and 24 h post‐CCl 4 ‐exposure. At 4 h, the RER became degranulated, mitochondrial eristae indistinct with electron dense flocculant matrieal deposits, and nuclear membranes indented. Autophagic vacuoles were present in some hepatocytes. Within 48 h post‐exposure, the hepatocytes were indistinguishable from control hepatocytes. CCl 4 can induce reversible or irreversible cell damage which appears dose dependent.