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Epizootic haematopoietic necrosis virus: detection by ELISA, immunohistochemistry and immunoelectron‐microscopy
Author(s) -
HYATT A. D.,
EATON B. T.,
HENGSTBERGER S.,
RUSSEL G.
Publication year - 1991
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1991.tb00619.x
Subject(s) - immunoelectron microscopy , rainbow trout , biology , immunohistochemistry , virology , virus , antibody , epizootic , pathology , fishery , immunology , fish <actinopterygii> , medicine
. Epizootic haematopoietic necrosis virus (EHNV) was isolated from cultured rainbow trout, Oncorhynchus mykiss (Walbaum). Antibodies to the virus and to associated capsid subunits have been produced and used in immunohistochemistry, immunoelectron‐microscopy and an antigcn‐capture‐immunosorbent assay (ELISA). The results show that both antibodies can be used by various immuno‐procedures to detect both redfin perch, Perca ftuviatilis L., and rainbow trout isolates of EHNV. The procedures described provide for the first time rapid and specific tests for the detection of EHNV in cultured and clinical material.