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Adherence, haemagglutination and cell surface characteristics of motile aeromonads virulent for fish
Author(s) -
CORRAL F.,
SHOTTS E. B.,
BROWN J.
Publication year - 1990
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1990.tb00782.x
Subject(s) - virulence , acriflavine , aeromonas hydrophila , agglutination (biology) , microbiology and biotechnology , biology , hemagglutination , fimbria , pilus , enterotoxin , catfish , bacteria , virology , fish <actinopterygii> , escherichia coli , antigen , gene , biochemistry , immunology , virus , genetics , fishery
. Motile Aeromonas spp. virulent for fish were studied with regard to their adhesion profile. Electron microscopy demonstrated the presence of fimbriae (pili) on Aeromonas cells regardless of virulence potential. The results show no significant correlations between ability to haemagglutinate, yeast cell co‐agglutination and virulence. All strains expressed mannose‐sensitive haemagglutinin activity against guinea pig erythrocytes. Using four types of red blood cells no characteristic haemagglutinin pattern related to virulence could be discerned. Expression of surface haemagglutin(s) on Aeromonas hydrophila appears to be medium dependent; strains grown in liquid media demonstrated enhanced haemagglutination activity. Both virulent and avirulent strains had in vitro epithelial cell adhesive capabilities. Cell surface characteristics measured by agglutination in acriflavine and stability after boiling indicated that most virulent strains agglutinated in the presence of acriflavine, but not all sedimented after boiling. The ability of 10 selected strains of A hydrophila to grow in normal pooled catfish serum was determined. Only 17% of the virulence variations can be explained by their sensitivity to serum.