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Nutritional requirements for extracellular accumulation of toxin produced by a pathogenic Vibrio sp.
Author(s) -
BROWN C.
Publication year - 1990
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1990.tb00779.x
Subject(s) - biology , crassostrea , microbiology and biotechnology , toxin , oyster , vibrio , bioassay , pathogen , biochemistry , metabolite , asparagine , hypoxanthine , histidine , extracellular , hatchery , bacteria , amino acid , fishery , ecology , fish <actinopterygii> , genetics , enzyme
. A Vibrio species isolated from hatchery‐reared, diseased oyster larvae, Crassostrea virginica Gmelin, was shown to produce a teratogenic metabolite when grown in a complex, undefined medium. Nutritional studies determined that the pathogen required several inorganic salts, glucose and asparagine for growth. Bioassays demonstrated that toxin did not accumulate in this chemically‐defined medium. However, toxin accumulation did occur when the medium was supplemented with hypoxanthine and either glutamic acid, histidine or thiosulphate.