Premium
Specific immunoglobulins in serum from Atlantic salmon, Salmo salar L., immunized with Vibrio salmonicida and infectious pancreatic necrosis virus
Author(s) -
HÅVARSTEIN L. S.,
ENDRESEN C.,
HJELTNES B.,
CHRISTIE K. E.,
GLETTE J.
Publication year - 1990
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1990.tb00763.x
Subject(s) - infectious pancreatic necrosis virus , vibrio anguillarum , biology , aeromonas salmonicida , yersinia ruckeri , salmo , serotype , antiserum , microbiology and biotechnology , antibody , immune system , virology , antigen , vibrio , virus , western blot , rainbow trout , immunology , bacteria , fishery , fish <actinopterygii> , gene , biochemistry , genetics
. Atlantic salmon, Salmo salar L., responded to intraperitoneal injection of formalin killed Vibrio salmonicida or live infectious pancreatic necrosis virus ( ipnv ) by producing specific antibodies. The antibody titre varied significantly within the group tested. Western blot analysis demonstrated that high‐titre antisera recognized two major bacterial antigens with molecular weights of 12–15 kD and 22–27 kD. In addition, a few narrow bands with higher molecular weights were observed. An antiserum raised against IPNV recognised two major antigens corresponding to the structural proteins of the virus. E lisa and Western blot analysis showed that the immune serum raised against Vibrio salmonicida reacted slightly with Vibrio anguillarum , whereas no reaction to Yersinia ruckeri or Aeromonas salmonicida was detected. Indirect elisa and an elisa competition assay revealed that the immune serum raised against the N1 serotype was specific for this serotype of ipnv . The results demonstrate that Atlantic salmon has a humoral immune system capable of producing antibodies which discriminate between related bacterial antigens and between different serotypes of a virus.