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Use of an indirect enzyme‐linked immunosorbent assay ( elisa ) in the detection of channel catfish, Ictalurus punctatus (Rafinesque), antibodies to Edwardsiella ictaluri
Author(s) -
WATERSTRAT P.,
AINSWORTH J.,
CAPLEY G.
Publication year - 1989
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1989.tb00280.x
Subject(s) - edwardsiella ictaluri , ictalurus , catfish , biology , antibody , microbiology and biotechnology , agglutination (biology) , titer , fish <actinopterygii> , virology , immunology , fishery
. The use of an indirect enzyme‐linked immunosorbent assay ( elisa ) for the detection of channel catfish antibody to Edwardsiella ictaluri is described. Changes in agglutination titre in fish immunized with Edwardsiella ictaluri heat killed whole bacterins or lipopolysaccharides were reflected by corresponding changes in elisa readings. Relatively high correlations were observed among elisa OD readings, computed elisa titres and corresponding agglutination titres.