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Histopathology, electron microscopy and isolation of channel catfish virus in experimentally infected European catfish, Silurus glanis L.
Author(s) -
CHUMGSITATHUM B.,
PLUMB J. A.,
HILGE V.
Publication year - 1988
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1988.tb01230.x
Subject(s) - catfish , histopathology , spleen , biology , virus , pathology , anatomy , virology , fish <actinopterygii> , fishery , immunology , medicine
Two groups of European catfish, Silurus glanis L., fingerlings were infected with channel catfish virus (CCV) by either intraperitoneal injection with 10 5 TCID 50 of CCV, or bathing in water containing 10 5 TCID 50 of CCV per 1·0 ml. The virus was isolated from spleen, intestine and brain of CCV‐injected fish at day 1 and the titres ranged from 10 2·1 to 10 3·3 TCID 50 /g. However, the tissue distribution of CCV was irregular and no virus was isolated after day 3 post‐exposure. In CCV‐bathed fish, the virus was isolated only from the liver of one specimen at day 3 post‐exposure. No clinical signs of CCV disease developed in any of the fish. Specimens in each regime from all sampling periods showed some minor histopathological changes, but there were no differences between treatments. Lesions included oedema and focal haemorrhage in the liver and the spleen was congested. Electron micrographs of tissue samples showed the presence of a few virus particles around the nuclei of kidney, spleen and intestinal cells, and in or around a myelinated nerve within the optic lobes of infected fish during the first 4 days of infection.