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Use of a discontinuous Percoll gradient technique for the separation of channel catfish, Ictalurus punctatus (Rafinesque), peripheral blood leucocytes
Author(s) -
WATERSTRAT P. R.,
AINSWORTH A. J.,
CAPLEY G.
Publication year - 1988
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1988.tb01224.x
Subject(s) - percoll , catfish , ictalurus , biology , peripheral blood , differential centrifugation , chromatography , centrifugation , buffy coat , peripheral , andrology , medicine , immunology , endocrinology , microbiology and biotechnology , biochemistry , fishery , chemistry , fish <actinopterygii>
The use of a discontinuous density Percoll gradient in the separation of channel catfish peripheral blood leucocytes is described. The procedure yielded fractions enriched in monocytes and lymphocytes at the 1·060–1·065 g/ml interface, lymphocytes at the 1·065–1·070 g/ml interface, and neutrophils at the 1·070–1·080 g/ml interface. Cell purities among fractions ranged from 14·4–51·6% for monocytes, 61·4–72·8% for lymphocytes, and 60·4–79·2% for neutrophils. Factors influencing the outcome of the separation procedure include the use of pooled blood samples and calcium‐ magnesium‐free salt solutions.