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Enzyme‐linked immunosorbent assay (ELISA) detection of infectious pancreatic necrosis virus (IPNV) in culture fluids and tissue homogenates of the rainbow trout, Salmo gairdneri Richardson
Author(s) -
RODÁK L.,
POSPÍŠIL Z.,
TOMÁNEK J.,
VESELÝ T.,
OBR T.,
VALÍČEK L.
Publication year - 1988
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1988.tb00543.x
Subject(s) - infectious pancreatic necrosis virus , rainbow trout , serial dilution , biology , immunodiffusion , antibody , ouchterlony double immunodiffusion , immunoperoxidase , virology , virus , microbiology and biotechnology , immunology , medicine , pathology , monoclonal antibody , fish <actinopterygii> , antiserum , alternative medicine , fishery
. A double antibody enzyme‐linked immunosorbent assay (ELISA) for the detection of infectious pancreatic necrosis virus (IPNV) is described. The sensitivity of the assay reached 10 2 TCID 50 per 0·1 ml of culture fluid. The specificity of anti‐IPNV sera and of the assay was confirmed by agar‐gel immunodiffusion, by the direct immunoperoxidase technique for the deletion of IPNV in tissue cultures and by the ELISA inhibition test. High values of specific inhibition (over 90% at serum dilutions 1:40–1:2560) and low values of non‐specific inhibition (8·4% at serum dilution 1:160) demonstrated the quality of the rabbit anti‐IPNV serum. The results of ELISA agreed well with those of virological examinations. The potential of ELISA for investigations of a large series of field samples is discussed.