Premium
Cell‐mediated protection in carp, Cyprinus carpio L., against Aeromonas hydrophila
Author(s) -
BABA T.,
IMAMURA J.,
IZAWA K.,
IKED K.
Publication year - 1988
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1988.tb00536.x
Subject(s) - carp , aeromonas hydrophila , cyprinus , biology , thymocyte , lipopolysaccharide , immune system , microbiology and biotechnology , immunity , macrophage , common carp , phagocytosis , immunology , t cell , fish <actinopterygii> , fishery , in vitro , biochemistry
. The mechanism of protection against Aeromonas hydrophila infection in carp was studied. Recipient fish, into which pronephric cells from carp previously immunized by dipping in bacterial crude lipopolysaccharide (LPS) at 25°C for 2h were transferred, demonstrated almost the same level of protective ability as an immunized control group. The protective ability was transmitted by non‐adherent (to nylon fibre) immune pronephric cells. These non‐adherent cells were damaged by anti‐carp thymocyte serum and were, thus, considered to be T‐like cells. The protective ability was depressed in immunized carp treated with anti‐carp thymocyte serum in vivo and was also remarkably reduced in immunized carp whose macrophage function was impaired by Dextran sulphate‐500 treatment. These results indicate that the protection shown by carp immunized by dipping in crude LPS is dependent on cellular immunity regulated by a T‐like cell‐macrophage system.