Premium
Mycoplasma contamination in fish cell lines: an evaluation of detection by differential incorporation of 3 H‐uridine and 14 C‐uracil
Author(s) -
SCHULTZ C. L.,
LIDGERDING B. C.,
McALLISTER P. E.,
HETRICK F. M.
Publication year - 1986
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1986.tb00990.x
Subject(s) - uracil , biology , uridine , rainbow trout , cell culture , contamination , microbiology and biotechnology , mycoplasma , fish <actinopterygii> , biochemistry , fishery , rna , ecology , genetics , gene , dna
. Differential incorporation of uridine and uracil was used to assay for mycoplasma contamination in five fish cell lines: bluegill fry (BF‐2), chinook salmon embryo (CHSE‐214), epithelioma papillosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG‐2). The method was not suitable for monitoring BF‐2, CHSE‐214, FHM, and RTG‐2 cell lines because they incorporated uracil. Differential incorporation of uridine and uracil may be applicable for screening EPC cells because only this cell line could distinguish cultures experimentally infected with Mycoplasma orale from cultures known to be free from microbial contaminants.