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Spring viraemia of carp virus (SVCV): comparison of immunoperoxidase, fluorescent antibody and cell culture isolation techniques for detection of antigen
Author(s) -
FAISAL M.,
AHNE W.
Publication year - 1984
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1984.tb00906.x
Subject(s) - immunoperoxidase , spleen , biology , antigen , virus , virology , carp , infectivity , antibody , immunofluorescence , kidney , titer , monoclonal antibody , immunology , fish <actinopterygii> , endocrinology , fishery
. The sensitivity of the immunoperoxidase (IP) and fluorescent antibody (FA) techniques applied to frozen sections of organs of carp infected with spring viraemia virus (SVCV) was similar, both in respect of the intensity of the reaction and in the detection rate of the antigen. Seven days after a waterborne infection both methods detected the antigen in the kidneys and to a lesser extent in the liver and spleen. Quantification of virus gave a titre of 10 2.8 to 10 5.5 TCID 50 /g of kidney, whereas the agent could not be isolated from liver and spleen tissue. In contrast, at 24 1 / 2 days post–infection the viral antigen could be readily detected in liver and spleen but only occasionally in the kidneys using the IP and FA techniques. At this time, liver and spleen showed infectivity titres of 10 3.8 to 10 7.2 TCID 50 /g tissue, whereas the kidneys were found to be free of infective virus. It is concluded that using the IP and FA techniques SVCV can be detected most frequently in the kidneys from 7 to 14 days post–infection and in the liver and spleen from 14 to 24 days post‐infection.