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Inactivation of infectious pancreatic necrosis virus for vaccine use
Author(s) -
DIXON P. F.,
HILL B. J.
Publication year - 1983
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1983.tb00095.x
Subject(s) - infectious pancreatic necrosis virus , antigenicity , virus , infectivity , virology , biology , incubation , antibody , microbiology and biotechnology , necrosis , inactivated vaccine , incubation period , immunology , biochemistry , genetics
. Formalin and β‐propiolactone (BPL) were compared for efficacy in inactivating infectious pancreatic necrosis virus (IPNV) for vaccine use. Incubation with a 1:200 dilution of formalin at 20°C for four days or longer, or a 1:200 dilution of BPL at 4°C for six days or longer, completely inactivated the virus infectivity. However, whereas treatment with formalin caused only a slight reduction in anti‐genicity as titrated by in vitro tests, treatment with BPL destroyed over 50% of the antigenicity of the virus. Virus inactivated with formalin also proved highly effective at inducing high titres of neutralizing antibody in trout.