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In vitro and in vivo passage of bacteria across restricted areas and isolated tissues of trout, Salmo trutta L. and S. gairdneri Richardson
Author(s) -
BOWERS A.,
ALEXANDER J. B.
Publication year - 1982
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1982.tb00467.x
Subject(s) - salmo , gill , biology , bacteria , microbiology and biotechnology , in vivo , brown trout , rainbow trout , infiltration (hvac) , zoology , trout , in vitro , anatomy , fishery , fish <actinopterygii> , genetics , physics , thermodynamics
. Isolated areas of the skin and gills of rainbow and brown trout were subjected to hyperosmotic infiltration with E. coli both in vitro and in vivo and examined for the passage of bacteria. Bacteria were recovered from the isolated gill arches only after treatment with balanced salt solution at x 10 concentration, followed by the bacterial suspension. All the skin flap experiments were negative. Bacteria were recovered from the blood offish when that part of the animal anterior to the pectoral fins was exposed to the infiltrating bacteria. They were only recovered when the animal was exposed to the full hyperosmotic infiltration schedule. The evidence strongly supports the view that the bacteria enter the animal through the gills.