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Effects of Acholeplasma laidlawii and an unidentified mycoplasma on selected fish cell cultures and the replication of fish viruses
Author(s) -
EMERSON M.,
NICHOLSON B. L.,
BAYER R.
Publication year - 1979
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1979.tb00162.x
Subject(s) - biology , microbiology and biotechnology , virology , mycoplasma , cytopathic effect , virus , necrosis , cell , biochemistry , genetics
. An unidentified mycoplasma was isolated from cultures of Atlantic salmon (AS) cells and implicated as the cause of cytopathic effects (CPE). The agent did not visibly affect RTG‐2 cells. Experimental infection of RTG‐2 cells with Acholeplasma laidlawii resulted in increased cellular granularity and destruction. Scanning electron microscopic (SEM) examination of infected RTG‐2 and AS cultures revealed typical mycoplasmas distributed on cell surfaces and a marked effect on the cell surface topography, characterized by a loss of microvilli and cellular processes. SEM also revealed mycoplasmal contamination that was not detected by culturing. Comparisons of contaminated and uncontaminated RTG‐2 cells showed enhanced (2–100 fold) replication of infectious pancreatic necrosis virus (IPNV) and infectious haematopoietic necrosis virus (IHNV) in the presence of A, laidlawii .