Premium
An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system
Author(s) -
GILBERT J. P.,
GRATZEK J. B.,
BROWN J.
Publication year - 1979
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1979.tb00158.x
Subject(s) - malachite green , tetrahymena pyriformis , in vitro , biology , serial dilution , pharmacology , toxicity , toxicology , tetrahymena , medicine , chemistry , biochemistry , pathology , alternative medicine , organic chemistry , adsorption
. An in vitro technique was developed to screen drugs for toxicity to laboratory cultured protozoans. Drugs were diluted using standard microtitration equipment. An easily cultured protozoan, Tetrahymena pyriformis , was added to the dilutions of drugs and viability was checked daily for 10 days. Drugs used as a model system to test the technique were formalin and malachite green. Effective concentrations of formalin determined by this in vitro technique were close to recommended therapeutic treatment levels for Ichthyophthirius . Malachite green was effective at concentrations in excess of recommended therapeutic treatment levels for Ichthyophthirius . The synergistic effect of formalin and malachite green was demonstrated. This technique can he of use in determining effectiveness of new drugs as parasiticides.