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Rapid and sensitive quantitation of morphine using HPLC with electrochemical detection
Author(s) -
Mashayekhi S. O.,
GhandforoushSattari M.,
Hain R. D. W.
Publication year - 2008
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.2008.00933.x
Subject(s) - morphine , chromatography , opioid , extraction (chemistry) , high performance liquid chromatography , chemistry , assay sensitivity , detection limit , solid phase extraction , pharmacology , medicine , biochemistry , pathology , receptor , alternative medicine
Summary Background: Morphine is one of the most widely used opioid analgesics for controlling pain in cancer and post‐operative patients. Objective: This study aimed at finding a sensitive method for measuring morphine. Method: A one‐step solid phase extraction was developed for extracting morphine from various samples, and morphine concentration was measured using a high‐performance liquid chromatographic system with electrochemical detection. The sensitivity of the assay was 1·53 ng/mL with a recovery of 93·4% ± 0·01. The mean intra‐assay and inter‐assay test for three concentrations was 10·54 and 7·47, respectively. The assay showed no cross‐reaction with a wide range of compounds. Conclusion: This method for morphine in small biological samples is easy, sensitive and reproducible with low cross‐reactivity.