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Molecular analysis of thiopurine S ‐methyltransferase alleles in Taiwan aborigines and Taiwanese
Author(s) -
Lu HF.,
Shih MC.,
Chang YS.,
Chang JY.,
Ko YC.,
Chang SJ.,
Chang JG.
Publication year - 2006
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.2006.00707.x
Subject(s) - thiopurine methyltransferase , methyltransferase , allele , pharmacogenetics , genetics , biology , pharmacology , medicine , genotype , azathioprine , methylation , gene , disease
Summary Background: Thiopurine S ‐methyltransferase (TPMT) is a cytosolic enzyme involved in the metabolism of these thiopurine drugs. Methylation of thiopurine drugs by TPMT competes with the formation of their active 6‐thioguanine nucleotide metabolite, thereby potentially modulating the therapeutic and toxic effects of these drugs. Objective: To analyze the thiopurine S ‐methyltransferase allelic frequencies in Taiwan aborigines and Taiwanese. Methods: We used polymerase chain reaction–restriction fragment length polymorphism method to determine the allelic frequencies of TPMT variants (TPMT*1–TPMT*8) in 409 Taiwan aborigines and 117 Taiwanese. Results and discussion: The results showed that the allelic frequencies of TPMT*1 were 99·88% and 98·72% for Taiwan aborigines and Taiwanese respectively. The allelic frequencies of TPMT*3C were 0·12% and 1·28% for Taiwan aborigines and Taiwanese respectively. No TPMT*2, 3A, 3B, 3D and 4–8 were found in these populations. Conclusion: Our results provide useful information for using thiopurine drugs in these populations.