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Genetic polymorphism of CYP2C8 in three Malaysian ethnics: CYP2C8*2 and CYP2C8*3 are found in Malaysian Indians
Author(s) -
Muthiah Y. D.,
Lee W. L.,
Teh L. K.,
Ong C. E.,
Ismail R.
Publication year - 2005
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.2005.00653.x
Subject(s) - cyp2c8 , biology , genetics , gene , genotype , cyp2c9
Summary Background: CYP2C8 is genetically polymorphic. Four variants, CYP2C8*2 , CYP2C8*3 , CYP2C8*4 and CYP2C8*5 , which contain mutations in the coding regions have been reported to exhibit different enzyme activity as compared with CYP2C8*1 . Objective: To determine the allele frequency of three codon‐changing variants ( CYP2C8*2 , CYP2C8*3 and CYP2C8*4 ) in the Malaysian population. Method: Healthy unrelated volunteers from three major races in Malaysia were recruited. The study was approved by the local Research Ethics Committee. DNA was extracted using a standard protocol. A two‐step multiplex PCR method was developed to detect three alleles of CYP2C8 . PCR results were confirmed by subsequent direct DNA sequencing. Result: Only the Indians showed CYP2C8 polymorphism with allele frequency of 98% for CYP2C8*1 , 0·8% for CYP2C8*2 and 1·2% for CYP2C8*3 . CYP2C8*4 was not detected in any of the ethnic groups. Conclusion: To the best of our knowledge, the current study described, for the first time polymorphisms of CYP2C8 in Malaysian Indians.