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Determination of ciprofloxacin concentrations in human serum and urine by HPLC with ultraviolet and fluorescence detection
Author(s) -
Sowinski K. M.,
Kays M. B.
Publication year - 2004
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.2004.00575.x
Subject(s) - chromatography , urine , chemistry , ciprofloxacin , high performance liquid chromatography , ultrafiltration (renal) , pharmacokinetics , reagent , pharmacology , medicine , antibiotics , biochemistry
Summary Objective: To develop a simple and sensitive high performance liquid chromatography method for the determination of ciprofloxacin concentrations in human serum and urine. Method: Serum proteins were removed by ultrafiltration through a filtering device after the addition of a displacing reagent. Urine samples were diluted with mobile phase prior to injection. Separation was achieved with a C18 reverse‐phase column and using ultraviolet (UVD) and fluorescence detection (FD) for serum samples and UVD for urine samples. Results: The quantitation limits of the assay were 20 ng/ml (FD) and 100 ng/ml (UVD) in serum and 1 μ g/ml in urine. The assay was successfully applied to a pharmacokinetic study of ciprofloxacin in healthy volunteers. Conclusion: The method presented for ciprofloxacin assay in human serum and urine requires less sample clean up and is more sensitive than those reported in the literature.