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Rapid micromethod for simultaneous measurement of oxcarbazepine and its active metabolite in plasma by high–performance liquid chromatography
Author(s) -
Matar K. M.,
Nicholls P. J.,
Al–Hassan M. I.,
Tekle A.
Publication year - 1995
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1995.tb00654.x
Subject(s) - chromatography , metabolite , active metabolite , chemistry , dichloromethane , detection limit , oxcarbazepine , pharmacokinetics , high performance liquid chromatography , acetonitrile , solvent , pharmacology , medicine , biochemistry , carbamazepine , organic chemistry , neuroscience , epilepsy , biology
SUMMARY A rapid method for the simultaneous determination of oxcarbazepine (OXC) and its active metabolite (10–hydroxycarbazepine) in human and rat plasma by reversed phase high–performance liquid chromatography is described. The method involves a simple one–step extraction of the drugs from plasma with dichloromethane. The extract was evaporated and the residue was reconstituted with mobile phase and injected onto a Novapak C 18 column. The eluting solvent was 20% aceto–nitrile in water at a flow rate of 1 5 ml/min and the detector was monitored at 215 nm. The detection limit of OXC and 10–hydroxycarbazepine was 50 and 20 ng/ml, respectively. The within–day and between–day coefficients of variation for OXC and its active metabolite were 2 57‐6 95% and 4 21‐8 3%, respectively. The relative and absolute recoveries varied between 71 4% and 104 0%. The applicability of the analytical procedure to pharmacokinetic studies was illustrated.