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Influence of background air on microbial contamination during simulated i.v.‐admixture preparation
Author(s) -
Doorne H.,
Bakker J. H.,
Meevis R. F.,
Marskamp A.
Publication year - 1994
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1994.tb00670.x
Subject(s) - contamination , syringe , air contamination , natural rubber , chemistry , waste management , medicine , biology , ecology , organic chemistry , psychiatry , engineering
SUMMARY The effect of the cleanliness of environmental air on the microbial contamination of a simulated i.v.‐admixture during its preparation by aseptic transfer was studied under three conditions: (i) in a laminar air flow (LAF) bench situated in a class 1000 clean room, (ii) in an LAF bench in a microbiology laboratory and (iii) on a bench in an ordinary laboratory. Three thousand bottles were manually filled with 10 ml of a nutrient broth under each of the three conditions. The liquid was transferred by means of a syringe and a needle that pierced through the rubber closures of the bottles. The numbers of contaminated bottles under those three conditions were 1, 1 and 2, respectively. Background contamination had no demonstrable effect on the incidence of contamination, provided that preparation of the simulated i.v.‐admixture solution was performed by skilled personnel in an LAF cabinet and air contact is avoided.