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Decontamination methods for cytotoxic drugs. 1. Use of a bioluminescent technique to monitor the inactivation of methotrexate with chlorine–based agents
Author(s) -
Wren A. E.,
Melia C. D.,
Gamer S. T.,
Denyer S. P.
Publication year - 1993
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1993.tb00579.x
Subject(s) - sodium hypochlorite , bioluminescence , chlorine , chemistry , human decontamination , methotrexate , mutagen , pharmacology , chromatography , biochemistry , biology , waste management , organic chemistry , immunology , engineering , dna
SUMMARY A new microbial bioluminescence assay has been used to monitor the loss of mutagenicity on inactivation of methotrexate by active chlorine–based agents. The drug was degraded to products that were non–active in this mutagen detection system, in agreement with previously described work. Presept granules appear to be a suitable alternative to sodium hypochlorite for inactivating solutions and surface spills of methotrexate. The bioluminescence assay appears to have potential for monitoring clean–up and decontamination procedures in areas where cytotoxic agents are used.