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SIMULTANEOUS HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF DAPSONE AND MONOACETYLDAPSONE IN HUMAN PLASMA AND URINE
Author(s) -
Abuirjeie M. A.,
Irshaid Y. M.,
AHadidi H. F.,
Rawashdeh N. M.
Publication year - 1991
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1991.tb00311.x
Subject(s) - urine , chromatography , dapsone , high performance liquid chromatography , chemistry , protein precipitation , urinary system , blood plasma , coefficient of variation , medicine , biochemistry , immunology
Summary A rapid, specific and a one‐stage protein precipitation method for simultaneous estimation of dapsone (DDS) and monoacetyldapsone (MAD) concentration in plasma and urine using high performance liquid chromatography (HPLC) is described. The applicability of the method for monitoring DDS and MAD blood levels in two different acetylator phenotype volunteers following the administration of 100‐mg oral dose of DDS was shown. Cumulative urinary excretion of DDS and MAD were studied in the same volunteers.