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KINETICS OF BACTERIAL GROWTH IN SIMPLE INTRAVENOUS SOLUTIONS AND ADMIXTURES
Author(s) -
Akpan U. E.,
Hugbo P. G.,
Nasipuri R. N.
Publication year - 1989
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1989.tb00262.x
Subject(s) - simple (philosophy) , kinetics , mathematics , chemistry , chromatography , physics , philosophy , classical mechanics , epistemology
Summary The growth of bacteria in intravenous solutions and admixtures has been studied under stationary conditions of incubation. All the solutions were inoculated with 100 organisms/ml, incubated at room temperature (27°C) or (37°C), with samples withdrawn at specified time intervals, and plated in quadruplicates. The simple intravenous (i.v.) solutions did not support significant growth ( P > 0·05) of any of the micro‐organisms. Growth in i.v. solutions containing 1 % blood was very significant ( P > 0·05), as demonstrated by the high apparent growth rate constants ( K ). The ratio of K for β‐lactamase producing bacteria (β‐lac + ) over that for non‐β‐lactamase producing bacteria (β‐lac ‐ ) was significant ( P < 0·05) at 37°C compared to that at 27°C. The higher K values for B. cereus in benzylpenicillin and cefuroxime solutions, respectively, compared to those in antibiotic‐free solutions, may be attributable to hydrolysis of the drugs, while the low K values for B. subtilis in the same solutions may be attributed to the inhibitory effects of the drugs. In conclusion, minute quantities of blood in i.v. solution tend to cause bacteria to multiply rapidly. The presence of β‐lactamase producing species might, in addition, hydrolyse susceptible β‐lactam antibiotics which are common additives to i.v. fluids.