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HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF THEOPHYLLINE IN SERUM AND ITS USE IN THERAPEUTIC DRUG MONITORING
Author(s) -
ElSayed Y. M.,
Islam S. I.
Publication year - 1989
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1989.tb00219.x
Subject(s) - theophylline , chromatography , dichloromethane , chemistry , acetonitrile , high performance liquid chromatography , elution , therapeutic drug monitoring , quantitative analysis (chemistry) , sodium acetate , drug , solvent , pharmacology , medicine , organic chemistry
Summary A rapid, highly sensitive high‐performance liquid chromatographic method has been developed for the determination of theophylline in serum using β‐hydroxyethyltheophylline as an internal standard (IS). Theophylline and IS were extracted from serum using a mixture of dichloromethane: isopro‐panol (90: 10, v/v) and eluted from a 5 μm, C‐18 reversed‐phase column at 60°C with a mobile phase consisting of sodium acetate buffer‐acetonitrile (90:10, v/v), at a flow rate of 1 ml/min with ultraviolet detection at 280 nm. Each analysis required no longer than 7 min to perform. Quantification was achieved by the measurement of the peak: height ratio and the relative and absolute recoveries varied from 86 to 100%. Within‐day coefficients of variation ranged from 2–9 to 51% and between‐day coefficients of variation from 11 to 5–9% in subtherapeutic, therapeutic, and toxic concentrations.