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COMPARATIVE STUDY BETWEEN LIQUID AND SOLID MEDIA FOR THE DETECTION OF BACTERIAL CONTAMINATION IN INTRAVENOUS SOLUTIONS
Author(s) -
Pappalardo G.,
Hirschi B.,
Pannatier A.,
Francioli P.
Publication year - 1988
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1988.tb00214.x
Subject(s) - contamination , chromatography , medicine , chemistry , biology , ecology
Summary For the sterility testing of intravenous solutions, the Pharmacopeia recommend the use of liquid media. However, contamination by a single microorganism during the test may lead to the inappropriate blocking of the release of the batch. An alternative method using solid media for culturing the membranes used in the filtration was therefore sought. With an artificial contamination with 10–100 colony‐forming‐units (cfu) of nine different micro‐organisms, including those advocated by the Pharmacopeia , the feasibility of two different methods was assessed using two different intravenous solutions manufactured in our hospital. The results show that recoveries of minute amounts of bacteria using solid media and liquid media were similar. In all experiments there was a concordance in both positive and negative results. However, the batches of solutions have to be tested as quickly as possible after their preparation, whatever the method used, to avoid false‐negative tests.