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HIGH‐PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF RANITIDINE IN PLASMA AND URINE
Author(s) -
Salem M. Shiekh,
Gharaibeh A. M.,
Alkaysi H. N.,
Badwan A.
Publication year - 1988
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1988.tb00204.x
Subject(s) - chromatography , urine , chemistry , ranitidine , dichloromethane , dilution , high performance liquid chromatography , ammonium acetate , medicine , solvent , pharmacology , biochemistry , physics , organic chemistry , thermodynamics
Summary A high‐performance liquid chromatographic method for the analysis of ranitidine in plasma and urine is described. Plasma samples were extracted with dichloromethane while urine samples were injected directly after dilution. The mobile phase consisted of: 0·05 m ammonium acetate buffer containing 0·01 m octane sulphonate, 5·3%; methanol, 31·6%; and acetonitrile, 63·1%. Detection was carried out at 330 nm. Metoclopramide was used as the internal standard. Peak height ratios were measured. Absolute recovery from plasma was 83–85%. Within and between day coefficients of variation ranged from 0·79 to 2·42% and 1·09–2·95% respectively. Plasma and urine samples from a healthy volunteer were analysed.