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SPECIFICITY OF A COLORIMETRIC PARACETAMOL ASSAY TECHNIQUE FOR USE IN CASES OF OVERDOSE
Author(s) -
Patel H. V.,
Morton D. J.
Publication year - 1988
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1988.tb00186.x
Subject(s) - absorbance , chromatography , colorimetry , colorimetric analysis , drug overdose , drug , chemistry , medicine , pharmacology , poison control , emergency medicine
Summary A simple, economical colorimetric procedure for the determination of Paracetamol in serum or plasma (50–450 μg/ml) is described. Using the described method it is possible to quantify Paracetamol concentrations within 25 min which makes the technique ideal for use in an emergency‐suspected overdose situation. A total of 178 drug/drug combination products were examined for possible interference with the method and of these, eight were shown to interfere significantly with the quantitative assay. However, in all cases it would be possible to correct for the interference to arrive at a semi‐quantitative value for Paracetamol concentration by using the absorbance and by comparing the visible colour of the test sample with the Paracetamol standards.