Premium
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF CEPHALEXIN IN SERUM AND URINE
Author(s) -
Najib N. M.,
Suleiman M. S.,
ElSayed Y. M.,
Abdulhameed M. E.
Publication year - 1987
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1987.tb00556.x
Subject(s) - chromatography , chemistry , urine , coefficient of variation , cephradine , elution , methanol , high performance liquid chromatography , quantitative analysis (chemistry) , antibiotics , cephalosporin , biochemistry , organic chemistry
SUMMARY A rapid, highly sensitive high performance liquid chromatographic method has been developed for the determination of cephalexin in serum and urine. Serum protein was precipitated with 1% zinc sulphate solution containing cephradine as the internal standard. The drugs were eluted from a 5 μm, C‐18 reversed‐phase column at ambient temperature with a mobile phase consisting of acetonitrile‐methanol‐acetate buffer of pH 4.2 (10:10:80%), at a flow rate of 1.4 ml/min with ultraviolet detection at 254 nm. Each analysis lasted 9 min. Quantification was achieved by the measurement of the peak‐height ratio and the relative and absolute recoverics varied from 98 to 103%. Detection limits for cephalexin were 1 μg/ml in serum and 5 μg/ml in urine. Within‐run coefficient of variation ranged from 0.73 to 5.63% at three different concentrations for the serum and urine assay.